CopyMinder can directly protect 32-bit and 64-bit programs.  However, it is possible to protect 16-bit programs by executing a 32-bit program from within them as follows:

1. Create a 32-bit program to do a protection check and protect it.
2. Execute this 32-bit program from your 16-bit or 64-bit program.
3. If there are any parameters that you need to pass back to the 16-bit  program, your 32-bit program should write these to the Windows Registry or a file.

Please note that this method is less secure than usual.